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Rev. argent. microbiol ; 38(4): 190-196, oct.-dic. 2006. ilus, tab
Article in Spanish | LILACS | ID: lil-634528

ABSTRACT

Se determinó la tipibilidad, la reproducibilidad y el poder discriminatorio de ERIC-PCR y ApaI-PFGE para establecer la relación genética de cepas de Pasteurella multocida. Se estudiaron 49 cepas de diferente origen, subespecie, biotipo, grupo capsular, serotipo somático y perfil de resistencia antimicrobiana. Por ERIC-PCR se establecieron 31 patrones, los que presentaron entre 10 y 14 bandas en un rango comprendido entre 0,2 y 1,2 kb. Por ApaI-PFGE se detectaron 37 patrones de restricción, los cuales presentaron entre 7 y 15 bandas bien definidas de 34 a 450 kb. La tipibilidad de ERIC-PCR fue del 100% (T=1) y la de ApaI-PFGE del 94% (T=0,94). La reproducibilidad de ambas técnicas fue del 100% (R=1); sin embargo, el poder discriminatorio de ERIC-PCR fue 93% (D=0,93) y el de ApaI-PFGE 98% (D=0,98). Mediante ambas técnicas fue posible agrupar las cepas con relación epidemiológica y diferenciar claramente las cepas no relacionadas. Se demostró el valor de ERIC-PCR y ApaI-PFGE para complementar estudios epidemiológicos, principalmente si las cepas en estudio son analizadas por ambas técnicas.


Typeability, reproducibility, and discriminatory power of ERIC-PCR and ApaI-PFGE to establish the genetic relation of P. multocida strains were determined. Forty-nine strains of different source, biotype, capsular group, somatic serotype, and resistance to antimicrobials were studied. By ERIC-PCR, 31 patterns were defined with 10 to 14 bands in a rank of 0.2 and 1.2 kb. By ApaI-PFGE, 37 restriction patterns were established with 7 to 15 bands of 34 to 450 kb. Typeability was 100% (T=1) for ERIC-PCR, and 94% (T=0.94) for ApaI-PFGE. Reproducibility of both techniques was 100% (R=1). Discriminatory power was 93% (D=0.93) for ERIC-PCR, and 98% (D=0.98) for ApaI-PFGE. By using both techniques, epidemiologically related strains were grouped, and unrelated strains were clearly differentiated. The value of ERIC-PCR and ApaI-PFGE as complements to epidemiologic studies was demonstrated, especially when both techniques were used to analyze the strains.


Subject(s)
Animals , Cattle , Humans , Electrophoresis, Gel, Pulsed-Field/methods , Polymorphism, Restriction Fragment Length , Pasteurella multocida/classification , Polymerase Chain Reaction/methods , Americas , Antarctic Regions , Australia , Bird Diseases/microbiology , Birds/microbiology , Cattle Diseases/microbiology , Chickens/microbiology , Deoxyribonucleases, Type II Site-Specific , DNA, Bacterial/genetics , Drug Resistance, Bacterial , Pasteurella Infections/microbiology , Pasteurella Infections/veterinary , Pasteurella multocida/genetics , Pasteurella multocida/isolation & purification , Poultry Diseases/microbiology , Reproducibility of Results , Swine Diseases/microbiology , Swine/microbiology , Turkeys/microbiology
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